The biodegradation of spent saline bottles, a low-density polyethylene product (LDPE) by two selected Arthrobacter sp. is reported in vitro conditions. Chemical and UV pretreatment play a vital role in enhancing the rate of biodegradation. Treated LDPE films exhibit a higher weight loss and density when compared to untreated films. Arthrobacter oxydans and Arthrobacter globiformis grew better in a medium containing pretreated films than in medium containing untreated films. The decrease in density and weight loss of LDPE was also more for pretreated film when compared to untreated films indicating the effect of abiotic treatment on mechanical properties of LDPE. The decrease in the absorbance corresponding to carbonyl groups and double bonds that were generated during pretreat-ment suggest that some of the double bonds were cut by Arthrobacter species. Since Arthrobacter sp. is capable of degrading urea, splitting of urea group were also seen in FTIR spectrum indicating the evidence of biodegradation after microbial incubation. The results of this study indicated that bio-degradation rate could be enhanced by exposing LDPE to calcium stearate (a pro-oxidant), which acts as photo inducer of UV irradiation. The present plastic disposal trend of waste accumulation can be minimized with this promising eco-friendly technique.