2. A total of 80 subjects were studied: 40 denture wearers and 40 non-denture wearers, matched by age and sex,. Each subject was instructed to perform oral rinsing using a phosphate-buffered saline solution, which was expectorated and processed for the recovery of Candida on Sabouraudûs dextrose agar. Isolates were speciated by culturing on chromogenic candida agar and noting species-specific colony characteristics.