Fig. 2. Agarose gel electrophoresis of amplification products of
PCR demonstrating the specificity of primers 261F and 261R.
Amplification of the 261 bp fragment was observed only in the
positive control and in MBV positive tissues. No cross reactivity of
primer 261F/261R was observed among other virus infected tissues
and healthy shrimp tissue. Lane M: 1 kb molecular weight ladder.
Lane 1: no-template (NT) control. Lane 2: positive control (clone
WS-1). Lanes 3 and 4: MBV positive tissues. Lanes 5–7: viral
infected tissues (IHHNV, WSSV, and BP respectively). Lane 8:
purified HPV virions. Lane 9: DNA extracted from SPF shrimp.
The data is representative of two independent experiments.