It may also be observed that the methods for the detection of Enterobacteriaceae were species-specific, i.e. unable to detect counts a strain not belonging to the Enterobacteriaceae (Staphylococcus aureus) family. They also demonstrated sensitivity since they also detected counts at the different contamination levels tested.
The specificity and sensitivity of the Petrifilm EB system can be explained by the detection principle of the method, with the plates using Violet Red Bile Glucose Agar (VRBG) as culture medium and 2, 3, 5-trifeniltetrazolium (TTC) as color indicator. This colorant is colorless in the oxidized form, and turns red when reduced by the mitochondrial succinate-desidrogenase enzyme of the microorganism, imparted by the formation of the formazan precipitate, which is a dark-colored product (Kornacki and Johnson, 2001).
It may also be observed that the methods for the detection of Enterobacteriaceae were species-specific, i.e. unable to detect counts a strain not belonging to the Enterobacteriaceae (Staphylococcus aureus) family. They also demonstrated sensitivity since they also detected counts at the different contamination levels tested.The specificity and sensitivity of the Petrifilm EB system can be explained by the detection principle of the method, with the plates using Violet Red Bile Glucose Agar (VRBG) as culture medium and 2, 3, 5-trifeniltetrazolium (TTC) as color indicator. This colorant is colorless in the oxidized form, and turns red when reduced by the mitochondrial succinate-desidrogenase enzyme of the microorganism, imparted by the formation of the formazan precipitate, which is a dark-colored product (Kornacki and Johnson, 2001).
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