SPF was determined in the samples in which the previous assay of organoleptic characteristic evaluation was performed, as well on days 1, 2, 5, 8, 12 and 15. To do so, samples were prepared according to the method proposed by Dutra et al.
(2004). In this manner, 0.5 g of each sample was mixed with an appropriate amount of distilled water to obtain a final concentration of 0.2 · 104 g/ml