Following centrifugation (13,000g, 15 min), the DNA pellet was washed with 70% (v/v) ethanol, air dried, resupended in 40 ll buffer (10 mM Tris–HCl, pH 8.0, 0.1 mM EDTA, pH 8.0) and stored at 4 C. The second method used was the Cetyl Trimethyl Ammonium Bromide (CTAB)