2.5. Cell culture
Melan-a cells were purchased from Wellcome Trust Functional Genomic Cell Bank (St. GeorgeMedical School, University
of London, England). Cells were grown in a 37 °C, 10% CO2 incubator in RPMI-1640 media supplemented with 10% fetal
bovine serum, 1% penicillin/streptomycin, 1% amphotericin B, 2 mM L-glutamine and 200 nM tetradecanoyl phorbol acetate
(TPA). Melan-a cells at passage 30 or less were used for the experiments [14].