Although several studies reported the development
of animal models with HPV infection12,13, the methods
used still have a lot of limitation and not practically
done. In short, (i) the difficulty in the preparation of
purified HPV viruses from infected tissue, (ii) the
need of human tissue as the starting materials used for
viral inoculation before transplanting it into an animal
and (iii) the beginning steps of tumor establishment
such as increase vascularization or angiogenesis could
not be observed in details. The advantages of the
dorsal skin-fold window chamber technique in
developing mouse baring HPV tumor are benefit for
the future study on testing any tumor-growth
inhibitorsespeciallyanti-angiogenesisdrug.
Moreover, the linear regression line will make it
simple to compare the efficacy of those inhibiting
agents. However, this technique always developed the
necrotizing area which appeared as hematoma lesion
but it is similar to previously reports of another
xenograft techniques14.
In conclusion, the present novel in vivo model for
HPV acquired tumor is simply and highly
reproducible in term of tumor induction model. This
kind of animal model may be useful for screening and
selecting candidate of novel anti-cancer agents in the
future.