Genetic analysis
Although rosettes and inflorescences of gsa-1 were similar to those
of Col-0 in morphology, its root displayed a defect in gravitropism
when grown on agar plates for 3 d after germination (DAG)
(Supporting Information Fig. S1). F1 and F2 progenies derived
from the crosses of Col-0 9 gsa-1 and Ler 9 gsa-1 were grown on
normal growth medium for 3 d; agravitropic individuals were
scored and compared statistically (v2 test). Segregation of the root
gravitropic phenotype of crossed F2 progeny seedlings at 5 DAG
on agar medium supplied with 30mM (NH4)2SO4 was also analyzed
and compared statistically.