Isolate AJS-15 was screened for their ability
to tolerate the abiotic stress particularly salt
and pH. A 20μl of overnight grown culture
was inoculated into nutrient broth amended
with 0.5% to 10% salt. After 24 h,
absorbance of the culture was determined at
600nm. The ability of the isolate to sustain
the pH was tested by growing in a varying
degree of pH from pH 5.0 to pH 13.0 in the
nutrient broth medium. Following the
growth of the isolate for 24 h, the
absorbance of the culture was taken at
600nm using uninoculated broth as a blank.
Isolate was inoculated in triplicate sets.