The reducing power assay was conducted as previously described by Wang et al. (2008) with ascorbic acid being used as the positive control. In brief, 2.5 ml of individual deionized water diluted lotus extract(ranged from 100 to 500 mg/ml) was sequentially mixed with(0.2 M, pH 6.6) and 1% equal volume of phosphate buffer potassium ferricyanide After incubation at 50°C for 20 min, 2.5 ml of 10% trichloroacetic acid was then added to the followed centrifuging at 3000 rpm for 10 min. Consequently, 5 ml of the upper layer was mixed with 2.5 ml of distilled water and 0.5 ml of ferric chloride(0.1%), while absorbance of the resulting solution was measured colorimetrically at 700 nm