agitation was applied
200–550 rpm and airflow 3–6 LPM. In addition to the
supply of air, pure oxygen (5%–10%) was also injected
whenever DO was found beyond the range. Active silicone
polymer antifoam A solution (Sigma) was added manually
on demand. Culture was induced with 0.5-mM IPTG at
optical density (OD) 1.025.