Analysis of carotenoids content
Carotenoid content was determined using acetone-petroleum ether extraction followed by spectrophotometric analysis,
as described by Rodriguez-Amaya, and Kimura (2004).
Extraction of carotenoids was performed by grinding the raw or boiled potatoes in a mortar and pestle,
filtration through a sintered glass filter under vacuum,
and separation of the carotenoids from acetone into petroleum ether.