For example, the period of time between the inoculation of fungal conidia and the application of active films should be
standardized because of germination during this period. The CFU method can provide only an evaluation of the effect of an active substance as compared to the control, because colonies can be obtained from one, many conidia, or fragments of the conidiophore or the mycelium. Care should also be taken while considering the shelf life of food products such as fruits when a large amount of conidia is inoculated. Usually, the number of fungal conidia responsible for spoilage is very low, sometimes as low as one conidium.