The seeds were placed to germinate in paper filter substrate previously moistened with sterile water, and packed in sterile Petri dishes (60 x 15 mm), and transferred to test tubes containing 20 mL of the MS culture medium (Murashige & Skoog, 1962),enriched with 8.88 µM of 6-benzylaminopurine (BAP). As source of explant during the subcultives, shoots produced by rhizome cultivated in vitro were used.