Production of ethanol from cassava starch
Ethanol was produced from cassava starch by the
method of Anyakorah et al. (1998), as presented in
Figure 2. The steps involved in the production were:
Gelatinization (50 kg cassava starch was stirred in
200 ml water at 80oC until smooth gel was formed);
Liquefaction (4 ml of α-amylase was added to
gelatinized starch at 80oC and incubated for 60
min); Saccharification (4 ml of aqueous solution
of amylo-glucosidase was added to liquefied starch at 60oC and incubated for 240 min); Filtration (the
hydrolysate was filtered through muslin cloth);
Fermentation (yeast inoculum was added to the
filtrate before anaerobic incubation at 28oC) and
Distillation (fermentation broth was filtered and
the filtrate passed through the distillation unit twice
at 90oC).