Although the determination of protein carbonyls is a routine
procedure for assessing protein oxidation, its application in foods
may overestimate the total amount of protein carbonyls due to
presence of artifacts, excess derivatization agent, and lipid-derived
carbonyls (Armenteros, Heinonen, Ollilainen, Toldrá, & Estévez, 2009).
Therefore, application of complementary techniques such as those based on the loss of sulphydryl groups might help to form a more
reliable picture of protein oxidation.