Filter Paper Activity (FPase) Assay For Cellulases
Descriptions: This protocol is a little modified but it is based on IUPAC procedure. Basically, this measures the ability of the cellulose to hydrolyze both crystalline and amorphous regions of cellulose. Therefore, it is generally believed that FPase assay is the best measure of the activities of both endo- and exo- type in a cellulase with a strip of filter paper (Whatman #1, 50mg, 1 X 6 cm) for 1hour reaction at the given temperature.