3.4. Study at optimum condition
After optimizing the conditions (NH4OH treatment as pre-treatment; particle size 45-63 μm; substrate loading
2.5gm/L; enzyme loading 30ml/L; pH 7; and temperature 35ºC) for Enzymatic hydrolysis of preris a further
reaction was studied and found the optimum sugar concentration 1.7625mg/L.
3.5. Fermentation of the reducing sugar solution
After separating the sugar solution from the remaining unreacted particle using filter and decolourising with
activated carbon the fermentation reaction was carried out with the Saccharomyces cerevisiae (Baker's yeast). For
this purposes 0.025gm, 0.05gm, and 0.075gm of the Saccharomyces cerevisiae was added in three reactor flask
having 75ml of the 1.7625mg/L concentrated reducing sugar solution. The other parameters were maintained
constant pH=7, and Temperature =25OC. The data collected time to time and found the total concentration of the
ethanol at 50 hours later is 0.333mg/L, which was the maximum concentration of ethanol for the study found for
0.075gm of yeast, and the others are 0.27mg/L for0.025gm yeast and 0.329mg/L for 0.05gm yeast which were seen
in the fig-4