Several studies on TDZ-induced regeneration have shown that TDZ can substitute for the auxin and cytokinin requirement of regeneration via somatic embryogenesis and shoot organogenesis in many species. In initial studies, TDZ was used in combination with other plant growth regulating substances. But later studies carried out in many plant species including tobacco, geranium, peanut confirmed the efficacy of TDZ as the sole growth regulating substance which could also simplifies the complex procedure of repeated subculturing on various combinations of PGRs (Murthy et al., 1998).