Significantly increased values were registered also in the presence of spruce bark extract – SB1, these being higher with 59.83% for Chl a, 78.16% for Chl b and 40.40% higher than control in the case of carotene pigment content. The utilization of spruce bark extracts (both SB2 andSB1) enhances the biosynthesis of assimilatory pigment accumulation in sunflower plants, and the differences can be correlated with polyphenolic extract concentrations.