Some of the reductases involved in reduction of Alamar Blue are present in subcellular components
and not only in the mitochondria [33,71] which implies that Alamar Blue reduction may not be entirely due to changes in mitochondrial function. Depending on the objectives of any given study, it may be necessary to determine the importance of mitochondria-restricted Alamar Blue reduction for each cell model. Knowing the contribution of mitochondrial reductases to Alamar Blue reduction may be important to establishing the predictive ability of in vitro data