Across experiments, no more than two animals per litter (one male
and one female) were assigned to each particular treatment. This
avoided overrepresentation of litters within each specific group. In
Experiments 1 and 2, each of the groups derived from the factorial
design was composed by 6 animals. Experimental groups in
Experiment 3 had a minimum of 5 and maximum of 8 animals.
Untreated control groups had 6 animals each, except for the female
group in Experiment 2, which had 5 subjects.