The assembly was cold-laminated using an 8 cm wide transparent adhesive tape. The laminated 26 × 8 cm assembly was cut into lateral- flow strips of 8 × 0.4 cm. The strips were stored in an airtight plastic bottle containing a desiccant pack. Extraction of TBSV-infected tomato Tomato leaf tissues infected by TBSV were extracted with 5 mL of 0.5X PBS (pH7.4) solution using tissue grinder (Agdia, USA). Then the crude sap was precipitated briefly and the supernatant was used for specificity of RIGS kit.