HPLC eluated compounds arrived to a
T-junction, where the methanolic DPPH solution with the concentration
of 6 × 10−5 mol/L was delivered via another LC pump with a
flow rate of 0.8 mL/min. After the eluates mixed with DPPH solution
in a reaction coil (10 m × 0.254 mm i.d. PEEK tubing), the negative
peaks were measured at 517 nm by DAD.