After thawing, the specimens were dissected into
different anatomical tissues, and then each tissue
was bioassayed according to the official guidelines
of the Japan Food Hygiene Association (2005).
Lethal potency was expressed in mouse units (MU)
where one MU is defined as the amount of toxin
required to kill a 20 g male mouse of ddY strain in
30 min after intraperitoneal injection.