The development of degradable and edible films from protein sources has drawn significant attention for the
utilisation of natural resources as well as for the alleviation of the environmental burden. Rice bran protein
(RBP) was applied to protein film preparation in this study. The protein solutions were casted on plastic
tissue culture dishes with glycerol as a plasticiser after heat treatment. Functional properties of the films were
then measured. The puncture strength (PS) of RBP films increased up to pH 8.0 and then decreased. PS of
protein films depends on the degree of protein purity, quality and composition. Higher concentration of
glycerol weakened the films. The pH affected the water solubility of RBP films and the films showed least
solubility at pH 3.0. RBP could be utilised in the preparation of degradable protein-based films. The RBPbased
film had functional properties comparable to those of the soy protein-based ones.