2.2. Plant material and extraction of Bougainvillea xbuttiana
The aerial part (flowers) of the plant was collected from
Cuernavaca (Mor. Mexico) in May 2010 and identified. A voucher
specimen (23683) is deposited at the Herbarium HUMO, CIByC
(UAEM). The extraction, fractionation and isolation of Bougainvillea
xbuttiana extract were performed as described (Patent MX/a/
2011/813522).
In brief, the flowers were cleansed using water and dried at
room temperature, powdered and extracted. The powdered
plant material (500 g) was macerated with ethanol 50% for
72 h at 25 1C. The extract was collected at room temperature,
filtered and evaporated to dryness under reduced pressure
in a rotary evaporator. The percentage yield was found to be
2.79 g. The ethanolic extract of Bougainvillea xbuttiana was
referred as Bxb.