I would like to let you know that at the moment I have some sequences from Cytochrom Oxidase I gene.
I did alignment my data with sequence belongs to Gwynne Shimin Lim in 2009 from GenBank. The Result show that sequences have many different conservation plot.
Perhaps because I used primer reference to Christoph Germann in 2011 including COI, 12S, 16S gene from mitochondria and 28S gene from Nucleus. Consequently, primer is not same. Now I have some problem because not found primer reference to Gwynne Shimin Lim in 2009. May I ask could you please give me some information about primer?