An obvious resemblance between the karyotypes of Ochotona huangensis and Ochotona dauurica was seen by the routine staining, despite of some bigger size of the first pair of Ochotona huangensis. The first four meta-submetacentric pairs of Ochotona dauurica are similar to the 2nd – 5-th pairs of Ochotona huangensis autosomes. The remaining five meta-submetacentric pairs of Ochotona dauurica, except the 10-th pair, are similar to the last five pairs of the first group of Ochotona huangensis autosomes. The 11-th – 15-th autosomes of Ochotona dauurica are very similar to the second subtelocentric group of Ochotona huangensis by morphology and sizes, with a loss of the little part of the upper arm on the 20-th pair. The absence of G-stained chromosomes not allows us to do unambiguous conclusion about the relationship between the karyotypes of Ochotona dauurica and Ochotona huangensis. Such species as Ochotona alpina (2n=42), Ochotona hyperborea (2n=40), Ochotona pallasi (2n=38) and Ochotona argentata (2n=38) of the subgenus Pika are close to Ochotona huangensis by the diploid chromosome number. However, they have more significant differences in relation of morphological groups and sizes of chromosomes.