In order to estimate the proportion of hookworm infections represented by Necator americanus and
Ancylostoma duodenale, the 2 major species of human hookworms in Nigeria, stool samples from 1253
hookworm-positive schoolchildren were cultured to the third-stage (L3), filariform larvae, using the
Harada-Mori test-tube method. N. americanus larvae were recovered from a total of 1177 (93.9%)
coprocultures while A. duodenale larvae were recovered from a total of 274 (21.9%) stool cultures.
58.2% of the hookworm infections were due solely to N. americanus, 6.1% solely to A. duodenale and
25.8% were mixed infections with both species. In all mixed infections, much higher number of N.
americanus larvae were recorded compared with those of A. duodenale.
In order to estimate the proportion of hookworm infections represented by Necator americanus andAncylostoma duodenale, the 2 major species of human hookworms in Nigeria, stool samples from 1253hookworm-positive schoolchildren were cultured to the third-stage (L3), filariform larvae, using theHarada-Mori test-tube method. N. americanus larvae were recovered from a total of 1177 (93.9%)coprocultures while A. duodenale larvae were recovered from a total of 274 (21.9%) stool cultures.58.2% of the hookworm infections were due solely to N. americanus, 6.1% solely to A. duodenale and25.8% were mixed infections with both species. In all mixed infections, much higher number of N.americanus larvae were recorded compared with those of A. duodenale.
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