Fig. 2. SDS–PAGE of purified amylase. Analysis was performed in gel containing
12% polyacrylamide. Electrophoresis was performed for over 75 min at 110 V at
room temperature. Protein band was visualized by Coomassie brilliant blue R-250.
M: Standard protein markers; lane 1: crude enzyme; lane 2: ammonium sujphateprecipitated
enzyme; lane 3: purified enzyme on Sephadex G-200 (gel filtration);
lane 4: purified enzyme on DEAE-Sephadex (ion-exchange).