Colon CSCs when treated with JPE at 30 µg/mL and 40 µg/mL respectively resulted in a dose-dependent suppression in colony formation (Figure 5A). Figure 5B also shows representative images collected from the colony forming assay and demonstrates the decreased colony number associated with JPE treatment compared to control. This shows that JPE affects colon CSCs self-renewal ability and thus demonstrates anti-cancer activities beyond suppressing proliferation and inducing apoptosis.