Each day same ration of feed for all experimental unitswas hand
loaded in the funnel of a feeder. Coupled with a timer, the feeder
released feed 2, 4 or 6 times a day at 12 h, 6 h or 4 h interval,
respectively, as designated in the experimental design. In this 4
week period, daily ration was gradually decreased from 5% to 3%
fish biomass as fish grew. A commercial tilapia feed was used,
which contained 25% crude protein, 3% crude fat, 12% crude ash,
6.5% crude fiber, 2% acid insoluble and 11% moisture. No water was
added or exchanged throughout the experiment.