The fatty acid composition of the SIO was
determined by GC. The oil was converted into
methyl esters (FAmE) and analyzed on an Agilent
model 7890A gas chromatograph (Santa Clara,
CA). The oven temperature was programmed as
follows: from 60°C (isothermal for 1 min) to 190°C
at 20°C/min, and isothermal period of 30 min at
190°C. The injector and detector temperatures
were set at 250°C. Helium was used as carrier gas.
GC separation peaks was performed on a BPX-
70 capillary column (60 m×0.25 mm i.d.×0.25 mm
film thickness; SGE, melbourne, Australia). Fatty
acids were identified by comparing their retention
times
with those of the FAmE standards purchased
from Sigma Aldrich (St. Louis, mO, USA) under
the same conditions. Peaks were integrated using
Agilent ChemStation software.
The fatty acid composition of the SIO was
determined by GC. The oil was converted into
methyl esters (FAmE) and analyzed on an Agilent
model 7890A gas chromatograph (Santa Clara,
CA). The oven temperature was programmed as
follows: from 60°C (isothermal for 1 min) to 190°C
at 20°C/min, and isothermal period of 30 min at
190°C. The injector and detector temperatures
were set at 250°C. Helium was used as carrier gas.
GC separation peaks was performed on a BPX-
70 capillary column (60 m×0.25 mm i.d.×0.25 mm
film thickness; SGE, melbourne, Australia). Fatty
acids were identified by comparing their retention
times
with those of the FAmE standards purchased
from Sigma Aldrich (St. Louis, mO, USA) under
the same conditions. Peaks were integrated using
Agilent ChemStation software.
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