.Preparation of Crude Extracts and Fish feed
The collected leaves were shade dried under normal environmental condition, ground into uniform powder using Thomas-Wiley machine. The powdered leaves of Ocimum basilicum (50g) were extracted by hydro-distillation method by using Soxhlet apparatus at room temperature. The filtrate was collected and the solvent was removed using rotary evaporator (Buchi SMP, Switzerland). The residue obtained after evaporation was dissolved and the desired amount of doses were prepared in sterile distilled water and stored at -200C until used for experimentation.