The important experimental factors that influence the outcomes of chemical mutagenesis include the concentration of mutagen; volume of mutagen relative to size of samples; treatment duration; temperature; pre-soaking of seeds; pH; use of catalytic agents; and the post-treatment handling of the putative mutants [39]. For physical mutagens, the authors [39] identified the presence of elements such as oxygen and chemical mutagenic agents; moisture content; impurities such as dust and fibers; and viral and bacterial infectious agents as being critical to the outcome. The determination of the optimal doses of mutagens and the dissociation of chimeras in putative mutants are probably the most critically important of factors for the success of induced mutations.