In thepresentstudyproteaseproducing

In thepresentstudyproteaseproducingbacterialstrainssuchas Bacillus cereus,Proteusmirabilis,Proteus
vulgaris and Enterobacter aerogenes wereisolatedfromspoiled fish andshrimptissues.Theisolated
strains wereindividuallyinoculatedintotheformulatedculturemediasuppliedwithvariousconcen-
trations (2.5–20%) oftrash fish (Odonus niger) meatpowderasmajorsubstrate.Amongthetested
substrateconcentrations,5%substratesuppliedmediumfavoredmaximumprotease(3.82–5.13IUml
1)
production. Afteroptimizationofsubstrateconcentration,theorganismsweretestedindividuallyfor
their proteaseproductioncapacityatvariouspHsandtemperatures.Theresultsshowedthatallthe
testedstrainswerecapableofproducinghighproteaseatpH6and50 1C. Thecrudeproteasesproduced
by thetestedorganismswereindividuallypurified byMLFTPPandASBPmethods.Thepartiallypurified
enzymes werescreenedagainstfewpathogenicbacterialstrains,anditwasobservedthattheenzyme
produced by P.vulgaris exhibitedthehighestantibacterialactivitywiththeinhibitionzoneranged
between 168.070.82 and204.371.25AU/ml.Further,thepartiallypurified enzymeof P.vulgaris alone
characterizedthroughSDS-PAGEanalysisanddetermineditsmolecularmassas65.0KDa.

ผลลัพธ์ (ไทย) 1:

ใน thepresentstudyproteaseproducingbacterialstrainssuchas Bacillus cereus, Proteusmirabilis, Proteusผดและ Enterobacter aerogenes wereisolatedfromspoiled ปลา andshrimptissues Theisolatedสายพันธุ์ wereindividuallyinoculatedintotheformulatedculturemediasuppliedwithvariousconcen-meatpowderasmajorsubstrate ปลา (ไนเจอร์ Odonus) oftrash trations (2.5 – 20%) Amongthetestedsubstrateconcentrations, substratesuppliedmediumfavoredmaximumprotease 5% (3.82 – 5.13IUml 1)การผลิต Afteroptimizationofsubstrateconcentration, theorganismsweretestedindividuallyforproteaseproductioncapacityatvariouspHsandtemperatures.Theresultsshowedthatallthe ของพวกเขาtestedstrainswerecapableofproducinghighproteaseatpH6and50 ค. 1 Thecrudeproteasesproducedโดย thetestedorganismswereindividuallypurified byMLFTPPandASBPmethods.Thepartiallypurifiedเอนไซม์ werescreenedagainstfewpathogenicbacterialstrains, anditwasobservedthattheenzymeผลิต โดย P.vulgaris exhibitedthehighestantibacterialactivitywiththeinhibitionzonerangedระหว่าง 168.070.82 and204.371.25AU/ml ต่อไป thepartiallypurified enzymeof P.vulgaris เพียงอย่างเดียวcharacterizedthroughSDS-PAGEanalysisanddetermineditsmolecularmassas65.0KDa

ผลลัพธ์ (ไทย) 2:

ใน thepresentstudyproteaseproducingbacterialstrainssuchas Bacillus cereus, Proteusmirabilis, Proteus
vulgaris และ Enterobacter aerogenes wereisolatedfromspoiled ปลา andshrimptissues.Theisolated
สายพันธุ์ wereindividuallyinoculatedintotheformulatedculturemediasuppliedwithvariousconcen-
trations (2.5-20%) ปลา oftrash (Odonus ไนเจอร์) Afteroptimizationofsubstrateconcentration, theorganismsweretestedindividuallyfor ของพวกเขา 1C Thecrudeproteasesproduced โดย thetestedorganismswereindividuallypurified byMLFTPPandASBPmethods.Thepartiallypurified werescreenedagainstfewpathogenicbacterialstrains เอนไซม์ anditwasobservedthattheenzyme ผลิตโดย P.vulgaris exhibitedthehighestantibacterialactivitywiththeinhibitionzoneranged ระหว่าง 168.070.82 and204.371.25AU / ml.Further, thepartiallypurified enzymeof P.vulgaris คนเดียวcharacterizedthroughSDS-PAGEanalysisanddetermineditsmolecularmassas65.0KDa

ผลลัพธ์ (ไทย) 3:

ใน thepresentstudyproteaseproducingbacterialstrainssuchas Bacillus cereus , proteusmirabilis ที่มี ,vulgaris และ Enterobacter aerogenes wereisolatedfromspoiled สเปคตรัม andshrimptissues ปลาwereindividuallyinoculatedintotheformulatedculturemediasuppliedwithvariousconcen - สายพันธุ์trations ( 2.5 – 20% ) oftrash ปลา ( odonus ไนเจอร์ ) amongthetested meatpowderasmajorsubstrate .substrateconcentrations , 5% substratesuppliedmediumfavoredmaximumprotease ( 3.82 ) 5.13iuml1 )การผลิต afteroptimizationofsubstrateconcentration theorganismsweretestedindividuallyfor ,theresultsshowedthatallthe proteaseproductioncapacityatvariousphsandtemperatures ของพวกเขาthecrudeproteasesproduced testedstrainswerecapableofproducinghighproteaseatph6and50 1C .โดย thetestedorganismswereindividuallypurified thepartiallypurified bymlftppandasbpmethods .werescreenedagainstfewpathogenicbacterialstrains anditwasobservedthattheenzyme เอนไซม์ ,ผลิตโดย p.vulgaris exhibitedthehighestantibacterialactivitywiththeinhibitionzonerangedระหว่าง 168.070.82 and204.371.25au / ml.further thepartiallypurified enzymeof p.vulgaris คนเดียว ,characterizedthroughsds-pageanalysisanddetermineditsmolecularmassas65.0kda .