Mosquitoes (Culicidae) are widespread insects and can be important in forensic context as a source of
human DNA. In order to establish the quantity of human DNA in mosquitoes’ gut after different post-
feeding interval and for how long after taking a bloodmeal the human donor could be identified, 174 blood-
engorged mosquitoes (subfamily Anophelinae and Culicinae) were captured, kept alive and sacrificed at 8 h
intervals. Human DNA was amplified using forensic PCR kits (Identifiler, MiniFiler, and Quantifiler). A full
DNA profiles were obtained from all Culicinae mosquitoes (74/74) up to 48 h and profiling was successful up
to 88 h after a bloodmeal. Duration of post-feeding interval had a significant negative effect on the
possibility of obtaining a full profile (p < 0.001), and logistic regression found that the probability of
obtaining the full profile is 15.5% less for every 8 h increase in post-feeding interval