Every extractive solution was collected and evaporated to dry at
40 C under reduced pressure with a rotary evaporator, respectively.
The residues obtained were dissolved with 20 mL solvent
and centrifuged for 10 min at 3000 rpm. Each solution was
collected and diluted with homologous solvent to a constant volume
for analysis. The solvent used was methanol for capsaicinoids
analysis, while acetone for red pigments analysis. HPLC analysis
was used for capsaicinoids, while colorimetric analysis for red
pigments.