These protocols have been used successfully for bacteria such as Escherichia coli,
Bacillus subtilis, Bordetella pertussis from nasopharyngeal swabs, Borrelia burgdorferi
from cerebrospinal fluid, and Legionella pneumophila from broncho-alveolar lavage.
For other bacteria, follow the protocol for Gram-positive bacteria, especially other
Gram-positive bacteria, which may be difficult to lyse
These protocols have been used successfully for bacteria such as Escherichia coli,Bacillus subtilis, Bordetella pertussis from nasopharyngeal swabs, Borrelia burgdorferifrom cerebrospinal fluid, and Legionella pneumophila from broncho-alveolar lavage.For other bacteria, follow the protocol for Gram-positive bacteria, especially otherGram-positive bacteria, which may be difficult to lyse
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