Substrateinduced
P. acidovorans (900 g) was suspended in 4 volumes of 10
mM potassium phosphate, pH 7, disrupted by ultrasonic oscillation
for 10 min, and centrifuged for 1 hour at 30,000 rpm to remove
cell debris. An aqueous solution of 5% streptomycin
sulfate, pH 7 (0.25 volume), was added to the supernatant and
after 15 min the mixture was centrifuged at 10,000 rpm; the
nucleic acid-streptomycin precipitate was discarded.
Ammonium Sulfate Fractionation-An equal volume