The Dixon method gave the best estimates of Ki for the strongest inhibitor (Ki 0.3) but only in two cases; 0.35 0.05 and 0.25 0.16 M from intersections of lines drawn at [S] 200 and 500 M with the line of [S] 1000 M, respectively. Furthermore, the Dixon method provides a range of Ki values that encompass zero
(i.e., negative values).
The ranges of the recovered values for KM and VMAX were rather
narrow and all ranges encompassed the correct value. Although the ranges were somewhat less variable for the SNLR method, the KM,app method provided essentially identical ranges. No attempt was made to estimate KM and VMAX from the Dixon analysis