tion; after collection of samples for chemical and toxicological analysis, the remaining effluent was used for the second step
treatment. During step 2 (AS treatment), 20 ml of sedimented AS were inoculated in the effluent (100 ml) coming from the six culture lines, after the fungal treatment. The experiment was performed in triplicates and flasks were incubated as described above for 48 h. At the end of the experiment, the biomass was filtered and dried for dry weight calculation.