Isolation and identification of
Enterobacteriaceae
For the isolation and identification of Enterobacteriaceae,
the buffered peptone water
was decanted and centrifuged at 11 000
rpm for 30 minutes in a refrigerated centrifuge.
The deposit was streaked on Salmonella/
Shigella agar (CM99), xylose–lysine
deoxycholate agar (Oxoid CM469) and
MacConkey agar (Oxoid CM7) for the detection
of Salmonella, Shigella, E. coli, and
other Enterobacteriaceae. All incubations
were at 37oC under aerobic conditions. Suspected
colonies were identified using standard
biochemical methods.