(0.1245 g, individual weight) were obtained and 14.35 g of dried
biomass was obtained from 500 BSFL grown on 500 g mixed feedstock
of biogas residue, the dryed BSFL biomass was placed into a
filter bag and soaked in petroleum ether (200 mL) for 48 h twice at
room temperature. Resulted leach liquor was combined. Crude larval
grease was then obtained by evaporating petroleum ether with
a rotary evaporator which was reported in Li et al. (Zheng et al.,
2012a). It yielded about 3.34 g of BSFL grease. Thereafter, grease
accounted for about 23.28% of the insect biomass as the comparison
with three kinds of manures described in Table 3. It proved
that biogas residue which was the second kind of waste after biogas
production by anaerobic fermentation could be utilized for
grease accumulation by BSFL.