Yeast immobilization is known to influence cellular metabolism
[1,2]. Cells of Saccharomyces cerevisiae entrapped
in calcium alginate, were shown to have a higher
glucose flux, more substrate was channeled to biomass and
ethanol [3]. Intracellular pH was found to be decreased,
whereas glucose phosphorylation was increased [4]. In addition,
the amounts of storage and structural carbohydrates
increased, as did the number of ploidy [5]. Due to the
decrease in growth rate, amino acid metabolism decreased
and increased concentrations of oxo-acids were released
into the fermentation medium