Results showed a lower survival of mixed cultures of R0052 and BB12 inoculated
at 1010 CFU/portion than survival values obtained at 108 CFU/portion.
Culturable and viable counts from sap inoculated at 1010 CFU/portion were significantly lower in sap diluted at 2 ºBx for every combination, highlighting the protective role of sap components at higher total soluble solids concentration.
The ability of maple sap concentrate to maintain probiotic viability was readily and accurately determined by PMA-qPCR.
According to this study, concentrated maple sap was able to maintain high viable and survival
counts of carefully selected probiotics, demonstrating its potential as fluid for the production of a probiotic beverage.