This provides an objective and spatially specific
representation of colocalization. ICA analysis generates a correlation/colocalization value for each
pixel defined by the Product of the Differences from the Mean (PDM) that is, PDM= (red intensitymean
red intensity) × (green intensity – mean green intensity). PDM values for each pixel can then be
visualized as an image showing positive intensity correlation (PDM>0) and negative intensity correlation
(PDM