Identification and Morphological Characteristics
Samples of rice plants with bakanae symptoms were collected and isolated on
pentachloronitrobenzene agar (PPA). Thirty-four single-spore isolates grown on
PDA (potato dextrose agar) and CLA (carnation leaf agar) plates were used for identification based on morphological characteristics. PDA was used to measure growth rate and pigmentation of culture, while CLA was used to determine shape and size of macroconidia and microconidia, conidiogenous cells and chlamydospore formation. Morphological characteristics on CLA were determined after 7–10 days of incubation under standard growth condition. The identification of Fusarium spp. was done basing on the taxonomic guidelines by Nirenberg and O’Donnell (1998) and Leslie and Summerell (2006).